Ganjiang Huangqin Huanglian Renshen Decoction improves insulin sensitivity by regulating intestinal flora in rats with Type 2 diabetes mellitus

Purpose: To study the effect of Ganjiang Huangqin Huanglian Renshen Decoction (GHHRD) on intestinal microflora and insulin resistance (IR) in type 2 diabetes mellitus (T2DM) rats. 
 Methods: Streptozotocin (STZ) injection was used to establish a rat model T2DM. The T2DM rats were divided into 5 groups: control, T2DM, + low-dose traditional Chinese medicine (TCM), high-dose TCM, metformin groups. Total DNA kit for extraction total from fecal microorganisms High-throughput 16S rDNA sequencing carried out identify flora Fasting plasma glucose (FPG) fasting serum (FINS) levels determined by biochemical analyzer. Results: analysis showed that after GHHRD treatment, Ruminococcus, Prevotellaceae, Escherichia-Shigella Bacteroidota tract exhibited significant changes. Furthermore, decreased Homeostasis Model Assessment-Insulin Resistance (HOMA-IR) index IR Conclusion: mitigates through alterations This provides theoretical basis potential use clinical management